Widespread Polycistronic Transcripts in Fungi Revealed by Single-Molecule mRNA Sequencing.
Identifieur interne : 000235 ( Main/Exploration ); précédent : 000234; suivant : 000236Widespread Polycistronic Transcripts in Fungi Revealed by Single-Molecule mRNA Sequencing.
Auteurs : Sean P. Gordon [États-Unis] ; Elizabeth Tseng [États-Unis] ; Asaf Salamov [États-Unis] ; Jiwei Zhang [États-Unis] ; Xiandong Meng [États-Unis] ; Zhiying Zhao [États-Unis] ; Dongwan Kang [États-Unis] ; Jason Underwood [États-Unis] ; Igor V. Grigoriev [États-Unis] ; Melania Figueroa [États-Unis] ; Jonathan S. Schilling [États-Unis] ; Feng Chen [États-Unis] ; Zhong Wang [États-Unis]Source :
- PloS one [ 1932-6203 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- génétique : ARN fongique, ARN messager, Agaricales.
- méthodes : Analyse de profil d'expression de gènes, Analyse de séquence d'ARN, Biologie informatique.
- Gènes fongiques, Phylogenèse.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : RNA, Fungal, RNA, Messenger.
- classification : Agaricales.
- genetics : Agaricales.
- methods : Computational Biology, Gene Expression Profiling, Sequence Analysis, RNA.
- Genes, Fungal, Phylogeny.
Abstract
Genes in prokaryotic genomes are often arranged into clusters and co-transcribed into polycistronic RNAs. Isolated examples of polycistronic RNAs were also reported in some higher eukaryotes but their presence was generally considered rare. Here we developed a long-read sequencing strategy to identify polycistronic transcripts in several mushroom forming fungal species including Plicaturopsis crispa, Phanerochaete chrysosporium, Trametes versicolor, and Gloeophyllum trabeum. We found genome-wide prevalence of polycistronic transcription in these Agaricomycetes, involving up to 8% of the transcribed genes. Unlike polycistronic mRNAs in prokaryotes, these co-transcribed genes are also independently transcribed. We show that polycistronic transcription may interfere with expression of the downstream tandem gene. Further comparative genomic analysis indicates that polycistronic transcription is conserved among a wide range of mushroom forming fungi. In summary, our study revealed, for the first time, the genome prevalence of polycistronic transcription in a phylogenetic range of higher fungi. Furthermore, we systematically show that our long-read sequencing approach and combined bioinformatics pipeline is a generic powerful tool for precise characterization of complex transcriptomes that enables identification of mRNA isoforms not recovered via short-read assembly.
DOI: 10.1371/journal.pone.0132628
PubMed: 26177194
PubMed Central: PMC4503453
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Schilling</name><affiliation wicri:level="2"><nlm:affiliation>Department of Bioproducts & Biosystems Engineering, University of Minnesota, Saint Paul, Minnesota, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Bioproducts & Biosystems Engineering, University of Minnesota, Saint Paul, Minnesota</wicri:regionArea><placeName><region type="state">Minnesota</region></placeName></affiliation></author><author><name sortKey="Chen, Feng" sort="Chen, Feng" uniqKey="Chen F" first="Feng" last="Chen">Feng Chen</name><affiliation wicri:level="2"><nlm:affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Energy Joint Genome Institute, Walnut Creek, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Wang, Zhong" sort="Wang, Zhong" uniqKey="Wang Z" first="Zhong" last="Wang">Zhong Wang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America; School of Natural Sciences, University of California at Merced, Merced, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America; School of Natural Sciences, University of California at Merced, Merced, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26177194</idno><idno type="pmid">26177194</idno><idno type="doi">10.1371/journal.pone.0132628</idno><idno type="pmc">PMC4503453</idno><idno type="wicri:Area/Main/Corpus">000246</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000246</idno><idno type="wicri:Area/Main/Curation">000246</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000246</idno><idno type="wicri:Area/Main/Exploration">000246</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Widespread Polycistronic Transcripts in Fungi Revealed by Single-Molecule mRNA Sequencing.</title><author><name sortKey="Gordon, Sean P" sort="Gordon, Sean P" uniqKey="Gordon S" first="Sean P" last="Gordon">Sean P. Gordon</name><affiliation wicri:level="2"><nlm:affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Energy Joint Genome Institute, Walnut Creek, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Tseng, Elizabeth" sort="Tseng, Elizabeth" uniqKey="Tseng E" first="Elizabeth" last="Tseng">Elizabeth Tseng</name><affiliation wicri:level="2"><nlm:affiliation>Pacific Biosciences, Menlo Park, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Pacific Biosciences, Menlo Park, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Salamov, Asaf" sort="Salamov, Asaf" uniqKey="Salamov A" first="Asaf" last="Salamov">Asaf Salamov</name><affiliation wicri:level="2"><nlm:affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Energy Joint Genome Institute, Walnut Creek, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Zhang, Jiwei" sort="Zhang, Jiwei" uniqKey="Zhang J" first="Jiwei" last="Zhang">Jiwei Zhang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Bioproducts & Biosystems Engineering, University of Minnesota, Saint Paul, Minnesota, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Bioproducts & Biosystems Engineering, University of Minnesota, Saint Paul, Minnesota</wicri:regionArea><placeName><region type="state">Minnesota</region></placeName></affiliation></author><author><name sortKey="Meng, Xiandong" sort="Meng, Xiandong" uniqKey="Meng X" first="Xiandong" last="Meng">Xiandong Meng</name><affiliation wicri:level="2"><nlm:affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Energy Joint Genome Institute, Walnut Creek, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Zhao, Zhiying" sort="Zhao, Zhiying" uniqKey="Zhao Z" first="Zhiying" last="Zhao">Zhiying Zhao</name><affiliation wicri:level="2"><nlm:affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Energy Joint Genome Institute, Walnut Creek, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Kang, Dongwan" sort="Kang, Dongwan" uniqKey="Kang D" first="Dongwan" last="Kang">Dongwan Kang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Energy Joint Genome Institute, Walnut Creek, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Underwood, Jason" sort="Underwood, Jason" uniqKey="Underwood J" first="Jason" last="Underwood">Jason Underwood</name><affiliation wicri:level="2"><nlm:affiliation>Pacific Biosciences, Menlo Park, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Pacific Biosciences, Menlo Park, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Grigoriev, Igor V" sort="Grigoriev, Igor V" uniqKey="Grigoriev I" first="Igor V" last="Grigoriev">Igor V. Grigoriev</name><affiliation wicri:level="2"><nlm:affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Energy Joint Genome Institute, Walnut Creek, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Figueroa, Melania" sort="Figueroa, Melania" uniqKey="Figueroa M" first="Melania" last="Figueroa">Melania Figueroa</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Pathology, University of Minnesota, Saint Paul, Minnesota, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Plant Pathology, University of Minnesota, Saint Paul, Minnesota</wicri:regionArea><placeName><region type="state">Minnesota</region></placeName></affiliation></author><author><name sortKey="Schilling, Jonathan S" sort="Schilling, Jonathan S" uniqKey="Schilling J" first="Jonathan S" last="Schilling">Jonathan S. Schilling</name><affiliation wicri:level="2"><nlm:affiliation>Department of Bioproducts & Biosystems Engineering, University of Minnesota, Saint Paul, Minnesota, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Bioproducts & Biosystems Engineering, University of Minnesota, Saint Paul, Minnesota</wicri:regionArea><placeName><region type="state">Minnesota</region></placeName></affiliation></author><author><name sortKey="Chen, Feng" sort="Chen, Feng" uniqKey="Chen F" first="Feng" last="Chen">Feng Chen</name><affiliation wicri:level="2"><nlm:affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Energy Joint Genome Institute, Walnut Creek, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author><author><name sortKey="Wang, Zhong" sort="Wang, Zhong" uniqKey="Wang Z" first="Zhong" last="Wang">Zhong Wang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America; School of Natural Sciences, University of California at Merced, Merced, California, United States of America.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America; School of Natural Sciences, University of California at Merced, Merced, California</wicri:regionArea><placeName><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agaricales (classification)</term><term>Agaricales (genetics)</term><term>Computational Biology (methods)</term><term>Gene Expression Profiling (methods)</term><term>Genes, Fungal (MeSH)</term><term>Phylogeny (MeSH)</term><term>RNA, Fungal (genetics)</term><term>RNA, Messenger (genetics)</term><term>Sequence Analysis, RNA (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN fongique (génétique)</term><term>ARN messager (génétique)</term><term>Agaricales (classification)</term><term>Agaricales (génétique)</term><term>Analyse de profil d'expression de gènes (méthodes)</term><term>Analyse de séquence d'ARN (méthodes)</term><term>Biologie informatique (méthodes)</term><term>Gènes fongiques (MeSH)</term><term>Phylogenèse (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Fungal</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Agaricales</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agaricales</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN fongique</term><term>ARN messager</term><term>Agaricales</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Computational Biology</term><term>Gene Expression Profiling</term><term>Sequence Analysis, RNA</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Analyse de séquence d'ARN</term><term>Biologie informatique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genes, Fungal</term><term>Phylogeny</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Gènes fongiques</term><term>Phylogenèse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Genes in prokaryotic genomes are often arranged into clusters and co-transcribed into polycistronic RNAs. Isolated examples of polycistronic RNAs were also reported in some higher eukaryotes but their presence was generally considered rare. Here we developed a long-read sequencing strategy to identify polycistronic transcripts in several mushroom forming fungal species including Plicaturopsis crispa, Phanerochaete chrysosporium, Trametes versicolor, and Gloeophyllum trabeum. We found genome-wide prevalence of polycistronic transcription in these Agaricomycetes, involving up to 8% of the transcribed genes. Unlike polycistronic mRNAs in prokaryotes, these co-transcribed genes are also independently transcribed. We show that polycistronic transcription may interfere with expression of the downstream tandem gene. Further comparative genomic analysis indicates that polycistronic transcription is conserved among a wide range of mushroom forming fungi. In summary, our study revealed, for the first time, the genome prevalence of polycistronic transcription in a phylogenetic range of higher fungi. Furthermore, we systematically show that our long-read sequencing approach and combined bioinformatics pipeline is a generic powerful tool for precise characterization of complex transcriptomes that enables identification of mRNA isoforms not recovered via short-read assembly. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26177194</PMID><DateCompleted><Year>2016</Year><Month>05</Month><Day>01</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>7</Issue><PubDate><Year>2015</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>Widespread Polycistronic Transcripts in Fungi Revealed by Single-Molecule mRNA Sequencing.</ArticleTitle><Pagination><MedlinePgn>e0132628</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0132628</ELocationID><Abstract><AbstractText>Genes in prokaryotic genomes are often arranged into clusters and co-transcribed into polycistronic RNAs. Isolated examples of polycistronic RNAs were also reported in some higher eukaryotes but their presence was generally considered rare. Here we developed a long-read sequencing strategy to identify polycistronic transcripts in several mushroom forming fungal species including Plicaturopsis crispa, Phanerochaete chrysosporium, Trametes versicolor, and Gloeophyllum trabeum. We found genome-wide prevalence of polycistronic transcription in these Agaricomycetes, involving up to 8% of the transcribed genes. Unlike polycistronic mRNAs in prokaryotes, these co-transcribed genes are also independently transcribed. We show that polycistronic transcription may interfere with expression of the downstream tandem gene. Further comparative genomic analysis indicates that polycistronic transcription is conserved among a wide range of mushroom forming fungi. In summary, our study revealed, for the first time, the genome prevalence of polycistronic transcription in a phylogenetic range of higher fungi. Furthermore, we systematically show that our long-read sequencing approach and combined bioinformatics pipeline is a generic powerful tool for precise characterization of complex transcriptomes that enables identification of mRNA isoforms not recovered via short-read assembly. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gordon</LastName><ForeName>Sean P</ForeName><Initials>SP</Initials><AffiliationInfo><Affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tseng</LastName><ForeName>Elizabeth</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Pacific Biosciences, Menlo Park, California, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Salamov</LastName><ForeName>Asaf</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Jiwei</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Bioproducts & Biosystems Engineering, University of Minnesota, Saint Paul, Minnesota, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meng</LastName><ForeName>Xiandong</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Zhiying</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kang</LastName><ForeName>Dongwan</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Underwood</LastName><ForeName>Jason</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Pacific Biosciences, Menlo Park, California, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Grigoriev</LastName><ForeName>Igor V</ForeName><Initials>IV</Initials><AffiliationInfo><Affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Figueroa</LastName><ForeName>Melania</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, University of Minnesota, Saint Paul, Minnesota, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schilling</LastName><ForeName>Jonathan S</ForeName><Initials>JS</Initials><AffiliationInfo><Affiliation>Department of Bioproducts & Biosystems Engineering, University of Minnesota, Saint Paul, Minnesota, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Feng</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Zhong</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Department of Energy Joint Genome Institute, Walnut Creek, California, United States of America; School of Natural Sciences, University of California at Merced, Merced, California, United States of America.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>07</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012331">RNA, Fungal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000363" MajorTopicYN="N">Agaricales</DescriptorName><QualifierName UI="Q000145" 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Gordon</name></region><name sortKey="Chen, Feng" sort="Chen, Feng" uniqKey="Chen F" first="Feng" last="Chen">Feng Chen</name><name sortKey="Figueroa, Melania" sort="Figueroa, Melania" uniqKey="Figueroa M" first="Melania" last="Figueroa">Melania Figueroa</name><name sortKey="Grigoriev, Igor V" sort="Grigoriev, Igor V" uniqKey="Grigoriev I" first="Igor V" last="Grigoriev">Igor V. Grigoriev</name><name sortKey="Kang, Dongwan" sort="Kang, Dongwan" uniqKey="Kang D" first="Dongwan" last="Kang">Dongwan Kang</name><name sortKey="Meng, Xiandong" sort="Meng, Xiandong" uniqKey="Meng X" first="Xiandong" last="Meng">Xiandong Meng</name><name sortKey="Salamov, Asaf" sort="Salamov, Asaf" uniqKey="Salamov A" first="Asaf" last="Salamov">Asaf Salamov</name><name sortKey="Schilling, Jonathan S" sort="Schilling, Jonathan S" uniqKey="Schilling J" first="Jonathan S" last="Schilling">Jonathan S. Schilling</name><name sortKey="Tseng, Elizabeth" sort="Tseng, Elizabeth" uniqKey="Tseng E" first="Elizabeth" last="Tseng">Elizabeth Tseng</name><name sortKey="Underwood, Jason" sort="Underwood, Jason" uniqKey="Underwood J" first="Jason" last="Underwood">Jason Underwood</name><name sortKey="Wang, Zhong" sort="Wang, Zhong" uniqKey="Wang Z" first="Zhong" last="Wang">Zhong Wang</name><name sortKey="Zhang, Jiwei" sort="Zhang, Jiwei" uniqKey="Zhang J" first="Jiwei" last="Zhang">Jiwei Zhang</name><name sortKey="Zhao, Zhiying" sort="Zhao, Zhiying" uniqKey="Zhao Z" first="Zhiying" last="Zhao">Zhiying Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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